[1]莫小锋,文清岚,秦丽萍,等.外源水杨酸和脱落酸复合处理提高杜鹃花叶片耐热性[J].北方园艺,2018,42(14):87-94.[doi:10.11937/bfyy.20174127]
　MO Xiaofeng,WEN Qinglan,QIN Liping,et al.Improving Heat-resistance of Rhododendron hybridum by Using Salicylic Acid Combined With Abscisic Acid During High Temperature Stress[J].Northern Horticulture,2018,42(14):87-94.[doi:10.11937/bfyy.20174127]
点击复制

外源水杨酸和脱落酸复合处理提高杜鹃花叶片耐热性

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年14 页码: 87-94 栏目: 出版日期: 2018-07-25
Title:
Improving Heat-resistance of Rhododendron hybridum by Using Salicylic Acid Combined With Abscisic Acid During High Temperature Stress
作者:
莫小锋1; 文清岚1; 秦丽萍1; 莫亿伟2; 蓝群3
（1.桂林市林业科学研究所，广西 桂林 541004；2.绍兴文理学院 生命科学学院，浙江 绍兴 312000；3.广西科技师范学院 食品与生化工程学院，广西 来宾 546100）
Author(s):
MO Xiaofeng1; WEN Qinglan1; QIN Liping1; MO Yiwei2; LAN Qun3
(1.Guilin Forestry Research Institute,Guilin,Guangxi 541004；2.College of Life Science,Shaoxing University,Shaoxing,Zhejiang 312000；3.College of Food and Biochemical Engineering,Guangxi Science and Technology University,Laibin,Guangxi 546100)
关键词:
杜鹃花; 高温胁迫; 水杨酸; 脱落酸; 抗氧化酶活性
Keywords:
Rhododendron hybridum; heat stress; salicylic acid; abscisic acid; antioxidant enzyme activity
DOI:
10.11937/bfyy.20174127
文献标志码:
A
摘要:
以生长一致2年生的“胭脂蜜”盆栽杜鹃花为试材，以喷施蒸馏水为对照(CK)，以水杨酸（SA）、脱落酸（ABA）及SA+ABA复合处理喷施2次叶片为处理，研究了SA和ABA处理对39 ℃/29 ℃高温胁迫下叶片抗氧化和耐热性的影响，以期通过喷施SA和ABA来提高杜鹃花耐热能力。结果表明：与对照相比，经SA、ABA和SA+ABA复合处理均可提高叶片可溶性总糖、脯氨酸含量、相对含水量和叶绿素含量，降低叶片的相对电导率；促进叶片超氧化物歧化酶(SOD)和过氧化氢酶（CAT）活性增加，降低叶片H2O2和细胞膜脂过氧化产物丙二醛（MDA）含量。但以SA+ABA复合处理对提高杜鹃花的耐热性效果最好，ABA次之，然后是SA。因此，SA+ABA复合处理更能促进渗透物质合成，提高抗氧化能力和细胞完整性，从而提高对高温胁迫抗性。
Abstract:
To explore the effects of exogenous salicylic acid (SA) and abscisic acid (ABA) treatment on heat resistance of Rhododendron hybridum.2-year-old Rhododendron hybridum ‘Yanzhimi’ were sprayed with 0 (CK),10.0 μmol·L-1 SA,30.0 μmol·L-1 ABA and 10.0 μmol·L-1 SA+30.0 μmol·L-1 ABA solution on the leaves and roots for two days,respectively.And then treated with heat stress (39 ℃/29 ℃,day/night) for 8 days by using plant growth chamber.The total chlorophyll content,osmotic regulation substances,membrane lipid peroxidation,and antioxidant enzymes activity of leaves were measured after different treatment times.The results showed that,compared with the control,treated with SA,ABA and SA+ABA could markedly increased total soluble sugar,proline,relative water and chlorophyll contents in leaves,and decreased relative electrical conductivity,malondialdehyde (MDA) and H2O2 content in leaves.Meanwhile,activities of SOD and CAT in leaves were improved.However,compared with the control,treated SA coupled with ABA had the best effect improving heat resistance for Rhododendron hybridum,and the next were treated with ABA and SA.These results showed that treated with SA+ABA could improve osmotic regulation substances content,inhibite water loss,chlorophyll degradation and membrane oxidation,enhance the resistance to high temperature stress and alleviate the negative injuries.

相似文献/References:

[1]王振兴,艾 军,杨义明,等.不同温度对五味子叶片光系统 Ⅱ 活性的影响[J].北方园艺,2014,38(14):160.
　WANG Zhen-xing,AI Jun,YANG Yi-ming,et al.Effect of Different Temperature Treatments on Photosystem Ⅱ in Leaves of Schisandra chinensis (Turcz.) Baill.[J].Northern Horticulture,2014,38(14):160.
[2]吴丽君,张俊凤.高温胁迫对番茄幼苗生长和生理指标的影响[J].北方园艺,2013,37(23):19.
　WU Li-jun,ZHANG Jun-feng.Effect of High Temperature Stress on Growth and Physiological Indexes of Tomato Seedlings[J].Northern Horticulture,2013,37(14):19.
[3]范飞,李绍鹏,高新生,等.不同茄子品种幼苗耐热性比较研究[J].北方园艺,2013,37(15):15.
　FAN Fei,LI Shao-peng,GAO Xin-sheng,et al.Comparative Study on the Heat Tolerance of Different Eggplant Varieties at Seedling Stage[J].Northern Horticulture,2013,37(14):15.
[4]朱鑫,沈火林.高温胁迫对芹菜幼苗细胞膜稳定性的影响[J].北方园艺,2014,38(07):15.
　ZHU Xin,SHEN Huo-lin.Effect of High Temperature Stress on Cell Membrane in Celery Seedlings[J].Northern Horticulture,2014,38(14):15.
[5]李荣华,郭培国,张华,等.高温胁迫对不同耐热性菜心材料生理特性的差异研究[J].北方园艺,2013,37(01):1.
　LI Rong-hua,GUO Pei-guo,ZHANG Hua,et al.Effects of Heat Stress on Several Physiological Traits in Heat-yolerant and Heat-Sensitive Genotypes of Flowering Chinese Cabbage[J].Northern Horticulture,2013,37(14):1.
[6]李荣华,郭培国,张华,等.高温胁迫对不同耐热性菜心材料生理特性的差异研究[J].北方园艺,2012,36(01):1.
　LI Rong-hua,GUO Pei-guo,ZHANG Hua,et al.Effects of Heat Stress on Several Physiological Traits in Heat-yolerant and Heat-Sensitive Genotypes of Flowering Chinese Cabbage[J].Northern Horticulture,2012,36(14):1.
[7]廖金,周泓,刘冰,等.夏季城市绿地中不同遮阳条件下杜鹃叶温变化及其生理响应[J].北方园艺,2013,37(07):74.
　LIAO Jin,ZHOU Hong,LIU Bing,et al.Influences of Different Shading on Leaf Temperature and Physiological Response to the Summer High Temperature of Rhododendron pulchrum[J].Northern Horticulture,2013,37(14):74.
[8]鲜小林,陈睿,秦帆,等.四川杜鹃花资源调查及其育种意义研究[J].北方园艺,2012,36(02):92.
　XIAN Xiao-lin,CHEN Rui,QIN Fan,et al.The Resources Investigation and Breeding Significance of Rhododendron in Sichuan[J].Northern Horticulture,2012,36(14):92.
[9]王文举,王振平.亚硒酸钠对“红地球”葡萄耐热性及果实品质的影响[J].北方园艺,2012,36(24):47.
　WANG Wen-ju,WANG Zhen-ping.The Effect of Sodium Selenite on Heat-resistance and Berry Quality of Red Globe Grape[J].Northern Horticulture,2012,36(14):47.
[10]王庆,刘安成,张瑞博,等.高温胁迫对多叶羽扇豆生长及生理指标的影响[J].北方园艺,2013,37(24):52.
　WANG Qing,LIU An-cheng,ZHANG Rui-bo,et al.Effects of High Temperature Stress on Growth and Physiological Indices of Lupinus polyphlla Lindl.[J].Northern Horticulture,2013,37(14):52.
[11]莫小锋,文清岚,秦丽萍,等.外源SA和ABA复合处理提高杜鹃花叶片耐热性[J].北方园艺,2018,42(12):1.[doi:10.11937/bfyy.20174127]
　MO Xiaofeng,WEN Qinglan,QIN Liping,et al.Improving Heat-resistance of Rhododendron hybridum by Using Salicylic Acid Combined With Abscisic Acid During High Temperature Stress[J].Northern Horticulture,2018,42(14):1.[doi:10.11937/bfyy.20174127]

备注/Memo

第一作者简介：莫小锋（1979-），男，本科，林业工程师，现主要从事园林树木抗性生理等研究工作。E-mail：156655266@qq.com.责任作者：莫亿伟（1971-），男，博士，副教授，现主要从事植物生理等研究工作。E-mail：455615189@qq.com.基金项目：浙江省自然科学基金资助项目（LY14C130003）。收稿日期：2018-02-01

更新日期/Last Update: 2018-08-07